APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human Dopamine Receptor D1 Intracellular domain, and it does not cross react with human DRD2 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human Dopamine Receptor D1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dopamine receptor D1 , DADR , DRD1A
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Cell projection, dendrite,Cell projection, dendritic spine
Background
This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX639344 WB Image
Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D1 antibody [HL2680] (GTX639344) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639344 IHC-P Image
Dopamine Receptor D1 antibody [HL2680] detects Dopamine Receptor D1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [HL2680] (GTX639344) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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